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rabbit Anti-CD31, BIO
點擊次數(shù):318 發(fā)布時間:2014-12-2
rabbit Anti-CD31, BIO conjugated
abcam
Catalog Number : RSF-0468R-BIO
Quantity size : 100ug (2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA and 0.1% Sodium azide. Reconstitute with 100ul sterile distilled water. )
background:
[FUNCTION]Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
Specificity : · anti-CD31 is a rabbit Polyclonal antibody,BIO conjugated
· specific for anti-CD31/BIO of Human, Mouse, Rat, Dog, Horse
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt:78kDa
Application :
WB=1:50:200
ELISA=1:100:1000
IHC-P=1:50-200
Flow-Cyt=1:50:200 IF=1:50-200
Excitation spectrum:540nm
Not yet tested in other applications
Optimal working dilutions must bedetermined by the end user
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.