SBI不含外泌體胎牛血清EXO-FBS-50A-1Exosome-depleted FBS
產(chǎn)品名稱(chēng):SBI不含外泌體胎牛血清EXO-FBS-50A-1Exosome-depleted FBS
產(chǎn)品規(guī)格:50ML,250ML,500ML
品牌:SBI system gibco
供應(yīng)商:栩冉生物
保存溫度:-20℃
運(yùn)輸條件:干冰
用途:適用外泌體研究,該產(chǎn)品僅供科研使用
特點(diǎn):無(wú)支原體,無(wú)內(nèi)毒素。
庫(kù)存狀態(tài):原裝,常備現(xiàn)貨
特惠價(jià)格:詢(xún)價(jià)
Exo-FBS Overview: When You're Not Studying Bovine Exosomes
Do you study bovine exosomes? If not, and you use fetal bovine serum (FBS) in your cell or tissue culture media, you may be isolating more bovine exosomes than you realize. Serum is a rich source of exosomes, FBS included. In order to help scientists study only the exosomes they want, SBI provides Exo-FBS™ exosome-depleted FBS.
- Exosome-sized vesicles removed
- Very low levels of CD63 positive cow exosomes
- Undetectable levels of cow microRNAs
- Comparable growth rates as standard FBS
- Identical use as standard FBS (add 10% in DMEM or RPMI)
SBI不含外泌體胎牛血清EXO-FBS-50A-1Exosome-depleted FBS
Background
Standard growth media for most cells in culture require FBS as a growth supplement to DMEM. FBS is derived from bovine (cow) serum and contains a high abundance of cow exosome vesicles. These exosomes can interfere or cause significant background issues when studying the exosomes secreted from your cells of interest in standard culture conditions.
SBI不含外泌體胎牛血清EXO-FBS-50A-1Exosome-depleted FBS
Conclusions
溫馨提示:該產(chǎn)品不可用于臨床治療。
當(dāng)外泌體在1980年*被發(fā)現(xiàn)后,其被認(rèn)為是細(xì)胞排泄廢物的一種方式,zui近的研究發(fā)現(xiàn)外泌體在很多生理病理上起著重要的作用,這些發(fā)現(xiàn)點(diǎn)燃了人們對(duì)細(xì)胞分泌膜泡的興趣。exosome及其他細(xì)胞外囊泡正起著*的細(xì)胞通訊作用。在腫瘤發(fā)展過(guò)程中,exosome介導(dǎo)了關(guān)鍵的步驟,如刺激血管生成,削弱免疫反應(yīng),甚至參與了轉(zhuǎn)移前微環(huán)境的形成。在正常的生理過(guò)程中,exosome也起了重要作用,如胎盤(pán)exosome幫助形成對(duì)母親免疫系統(tǒng)的免疫抑制屏障??傊?,在正常和病理的條件下,細(xì)胞都釋放小囊泡,以各種方式影響其他細(xì)胞。
外泌體去除血清 EXO-FBS-50A-1|血清中外泌體的提取方法
1.血清離心純化:3000g,10mins,取上清液。
2. 按下表加入血清和提取試劑盒,混勻,靜置30mins。
3. 離心:1500g,30mins,去上清液。
4. 純化離心:1500g,5mins,去上清液。
5. 加入30無(wú)RNA酶的水,吹打,放于-80凍存
無(wú)外泌體血清可以特異性激發(fā)一些細(xì)胞反應(yīng),表明它們可以在作為治療遞送載體方面有巨大的潛力。無(wú)外泌體血清的囊泡性質(zhì)使得它們適合作為用于藥物或核酸遞送的潛在納米載體。在這里,研究人員需要解決的問(wèn)題是,無(wú)外泌體血清的大小分布也會(huì)影響外泌體的治療潛力。1:利用聚合物沉淀分離的外泌體比超離法的粒子分布要小。2:劃痕實(shí)驗(yàn)證實(shí)聚合物沉淀所得的小粒徑的外泌體被細(xì)胞攝取后遷移更快。
公司與無(wú)外泌體血清 外泌體去除血清 EXO-FBS-50A-1 相關(guān)產(chǎn)品
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隨貨會(huì)提供詳細(xì)質(zhì)檢報(bào)告
外泌體去除血清Exosome-free FBS,來(lái)自國(guó)外*胎牛血清,國(guó)內(nèi)技術(shù)水平生產(chǎn),*。